Journal: International immunopharmacology
Article Title: Kaempferol improves mitochondrial homeostasis via mitochondrial dynamics and mitophagy in diabetic kidney disease.
doi: 10.1016/j.intimp.2025.115121
Figure Lengend Snippet: Fig. 4. Kaempferol improves mitochondrial dynamics and biogenesis in DKD rats. (A) Representative immunohistochemical staining images of dynamin-related protein 1 (Drp1), fission 1 (Fis1), mitofusin 1 (MFN1), and optic atrophy 1 (OPA1) (400× magnification). (B) Semiquantification of immunohistochemical stain ing for Drp1, Fis1, MFN1, and OPA1 expression (n = 6, data are presented as means ± SD). (C) Western blot analysis of protein expression levels of Drp1, Fis1, MFN1, and OPA1. (D) Relative expression levels of Drp1, Fis1, MFN1, and OPA1 (n = 4, data are presented as means ± SD). (E) Western blot analysis of PGC1α and TFAM expression levels. (F) Quantitative analysis of PGC1α and TFAM protein levels in different groups (n = 4, data are presented as means ± SD).
Article Snippet: Antibodies are as follows: Transforming growth factor-β (TGF-β) (21898–1-AP), α-smooth muscle actin (α-SMA) (14395–1-AP), collagen-IV (Col-4) (19674–1-AP), Bcl-2-associated X protein (Bax) (50599–2-Ig), B-cell lymphoma 2 (Bcl2) (26593–1-AP), Dynamin-1-like protein (Drp1) (12957–1-AP), fission 1 (Fis1) (10956–1-AP), Mitofusin-1 (MFN1) (13798–1-AP), Optic Atrophy 1 Protein (OPA1) (27733–1-AP), Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC1α) (66369–1-Ig), and β-actin (66009–1-Ig) were bought from Proteintech (Wuhan, China); microtubule-associated protein I light chain 3 (LC3) (ab192890), Beclin1 (ab207612), Autophagy-related 5 (ATG5) (ab108327), and p62 (ab109012) were bought from Abcam (Shanghai, China); Cysteineaspartic acid protease-3 (caspase-3) (9662) was bought from Cell Signaling Technology (Shanghai, China); Mitochondrial Transcription Factor A (TFAM) (A3173) was bought from ABclonal (Wuhan, China).
Techniques: Immunohistochemical staining, Staining, Expressing, Western Blot
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